UBQ-1 NHS Ester

Catalog#

Unit Size

Price (USD)

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Qty

Cat.#: 1731-5

Unit size: 5 mg

$105.00

in stock

Cat.#: 1731-25

Unit size: 25 mg

$325.00

in stock

Cat.#: 1731-100

Unit size: 100 mg

$895.00

in stock

Universal Black 1 Quencher (UBQ-1, Black Hole Quencher®-2 (BHQ®-1) equivalent) is a dark quencher with no native emission due to the polyaromatic-azo backbone. This nonfluorescent acceptor is often the quencher of choice for qPCR probes and for other fluorescence-quenched probe applications. A broad and intense quenching range from 480-580 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with green to yellow emitting dyes such as FAM, Oregon Green®, TET, JOE and HEX, Alexa Fluor® 488 and DyLight® 488.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

Specifications
Structure
Abs/Em Spectra
Protein Labeling Calculator
Documents

Reactivity

Primary amine

Abs maximum

534 nm

Extinction coefficient

34,000 cm-1M-1

Solubility

DMSO, DMF

Spectrally compatible dyes

FAM, Oregon Green®, TET, JOE and HEX, Alexa Fluor® 488 and DyLight® 488

Molecular weight

586.58

Storage conditions

-20C

Shipping conditions

Ambient temperature

Protein name

Protein molecular weight (Da)

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