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SY-9 NHS ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1706-1
Unit size: 5 mg
$95.00
in stock
Cat.#: 1706-25
Unit size: 25 mg
$295.00
in stock
Cat.#: 1706-100
Unit size: 100 mg
$895.00
in stock

SY-9 (QSY®-9 equivalent) is a more hydrophilic version of the popular SY-7 dark quencher (QSY®-7 equivalent). This nonfluorescent acceptor dye is often used for the preparation of peptide FRET probes. A broad and intense quenching range from 500-600 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with dyes emitting at 500 nm to 600 nm, such as Fluorescein, Oregon Green, Carboxyrhodamine 110 (Rhodamine Green), Alexa Fluor® 488, 532, 546, 555, 568, Cy3, TAMRA and ROX dyes.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

Reactivity
Primary amine
Abs maximum
560 nm
Extinction coefficient
85,000 cm-1M-1
Solubility
DMSO, DMF
Spectrally compatible dyes
Carboxyrhodamine 110 (Rhodamine Green), Alexa Fluor® 488, 532, 546, 555, 568, Cy3, TAMRA and ROX
Molecular weight
914.97
Storage conditions
-20C
Shipping conditions
Ambient temperature

spectrum

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

QSY® is a trademark of a registered trademark of Thermo Fisher Scientific.