Sulfo Cy5 DBCO is a bright, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes. This far-red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10.
Sulfo Cy5 DBCO is an azide-reactive probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. Sulfo Cy5 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Sulfo Cy5 DBCO is an ideal alternative to copper requiring fluorescent alkynes.