PB NHS Ester

Catalog#: 1245-5
Unit size: 5 mg
$139.00
In stock
Catalog#: 1245-25
Unit size: 25 mg
$395.00
In stock
Catalog#: 1295-100
Unit size: 100 mg
$1,295.00
In stock

PB dye (Pacific Blue™) is a bright, blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. The excitation and emission spectrum of PB dye offers minimal overlap with green fluorophore emission spectra, making it an ideal choice for the first violet channel. Conjugates of this dye are strongly fluorescent even at neutral pH.

The amine-reactive PB NHS Etser (Pacific Blue™ succinimidyl ester) can be used to can be used to create blue-fluorescent bioconjugates with excitation/emission maxima ~410/455 nm that are excitable by the 405 nm spectral line of the blue diode (violet) laser. NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond

PB NHS Ester is structurally identical to Pacific Blue™ Succinimidyl Ester sold by ThermoFisher Scientific.

Reactivity
Primary amine
Abs/Em Maxima
416/451 nm
Extinction coefficient
30,000 cm-1M-1
Solubility
DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 405, DyLight® 405, Cascade Blue
Molecular weight
339.21
Storage conditions
-20C
Shipping conditions
Ambient temperature

 

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Pacific Blue and Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.