PB dye (Pacific Blue™ equivalent) is a bright, blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. The excitation and emission spectrum of PB dye offers minimal overlap with green fluorophore emission spectra, making it an ideal choice for the first violet channel. Conjugates of this dye are strongly fluorescent even at neutral pH.
Medium length, hydrophobic spacer arm (total length is 22.4 angstroms) provides an adequate separation between the dye and peptide. Spacers of different lengths are available upon request.
PB is structurally identical to Pacific Blue™.