MB 594 NHS Ester

Catalog#

Unit Size

Price (USD)

Availability

Qty

Cat.#: 1646-1

Unit size: 1 mg

$129.00

in stock

Cat.#: 1646-5

Unit size: 5 mg

$395.00

in stock

Cat.#: 1646-25

Unit size: 25 mg

$1,295.00

in stock

Cat.#: 1646-100

Unit size: 100 mg

$2,495.00

in stock

MB 594 NHS Ester is a very hydrophilic, water-soluble red fluorescent dye. Structurally related to Alexa Fluor® 594 dye, MB™ 594 dye contains an additional, negatively charged sulfo group (SO3H) to improve water solubility and minimize self-quenching. Characteristic features of MB™ 594 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™ 594 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.

The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

Specifications
Structure
Abs/Em Spectra
Protein Labeling Calculator
Documents

Reactivity

Primary amine

Abs/Em Maxima

605/635 nm

Extinction coefficient

110,000 cm-1M-1

Solubility

Water, DMSO, DMF

Spectrally similar dyes

Alexa Fluor® 594, CF® 594, Texas Red

Molecular weight

941.01

Storage conditions

-20C

Shipping conditions

Ambient temperature

Protein name

Protein molecular weight (Da)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

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Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) *
* Leave blank or 0 if not used

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Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)