MB 594 Maleimide

Catalog#: 1648-1
Unit size: 1 mg
$129.00
Out of stock
Catalog#: 1648-5
Unit size: 5 mg
$395.00
Out of stock
Catalog#: 1648-25
Unit size: 25 mg
$1,295.00
Out of stock
Catalog#: 1648-100
Unit size: 100 mg
$2,495.00
Out of stock

MB™ 594 is a very hydrophilic, water-soluble red fluorescent dye. Structurally related to Alexa Fluor® 594 dye, MB™ 594 dye contains an additional, negatively charged sulfo group (SO3H) to improve water solubility and minimize self-quenching. Characteristic features of MB™ 594 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™ 594 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

Reactivity
Thiol (Cys)
Abs/Em Maxima
601/623 nm
Extinction coefficient
110,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 594, CF® 594, Texas Red
Molecular weight
996.06
Storage conditions
-20C
Shipping conditions
Ambient temperature

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