MB 543 NHS ester

Catalog#: 1631-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1631-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1631-25
Unit size: 25 mg
$1,295.00
In stock
Catalog#: 1631-100
Unit size: 100 mg
$2,495.00
In stock

MB™ 543 is a very hydrophilic, water-soluble orange fluorescent dye. Structurally related to Alexa Fluor® 546 dye, MB™ 543 dye contains an additional, negatively charged sulfo group (SO3H) to improve water solubility and minimize self-quenching. Characteristic features of MB™ 543 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™ 543 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.

The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

Reactivity
Primary amine
Abs/Em Maxima
543/566 nm
Extinction coefficient
105,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 546, CF® 543, TAMRA
Molecular weight
916.68
Storage conditions
-20C
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label