MB 543 NHS ester

Catalog#

Unit Size

Price (USD)

Availability

Qty

Cat.#: 1631-1

Unit size: 1 mg

$129.00

in stock

Cat.#: 1631-5

Unit size: 5 mg

$395.00

in stock

Cat.#: 1631-25

Unit size: 25 mg

$1,295.00

in stock

Cat.#: 1631-100

Unit size: 100 mg

$2,495.00

in stock

MB™ 543 is a very hydrophilic, water-soluble orange fluorescent dye. Structurally related to Alexa Fluor® 546 dye, MB™ 543 dye contains an additional, negatively charged sulfo group (SO3H) to improve water solubility and minimize self-quenching. Characteristic features of MB™ 543 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™ 543 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.

The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

Specifications
Structure
Abs/Em Spectra
Protein Labeling Calculator

Reactivity

Primary amine

Abs/Em Maxima

543/566 nm

Extinction coefficient

105,000 cm-1M-1

Solubility

Water, DMSO, DMF

Spectrally similar dyes

Alexa Fluor® 546, CF® 543, TAMRA

Molecular weight

916.68

Storage conditions

-20C

Shipping conditions

Ambient temperature

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