MB 543 Maleimide

Catalog#: 1638-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1638-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1638-25
Unit size: 25 mg
$1,295.00
In stock
Catalog#: 1638-100
Unit size: 100 mg
$2,495.00
In stock

MB™ 543 is a very hydrophilic, water-soluble orange fluorescent dye. Structurally related to Alexa Fluor® 546 dye, MB™ 543 dye contains an additional, negatively charged sulfo group (SO3H) to improve water solubility and minimize self-quenching. Characteristic features of MB™ 543 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™ 543 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

Reactivity
Thiol (Cys)
Abs/Em Maxima
543/566 nm
Extinction coefficient
105,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 546, CF® 543, TAMRA
Molecular weight
941.23
Storage conditions
-20C
Shipping conditions
Ambient temperature

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