MB 543 DBCO

Catalog#: 1635-1
Unit size: 1 mg
$139.00
In stock
Catalog#: 1635-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1635-25
Unit size: 25 mg
$1,395.00
In stock
Catalog#: 1635-100
Unit size: 100 mg
$2,795.00
In stock

MB™ 543 is a very hydrophilic, water-soluble orange fluorescent dye. Structurally related to Alexa Fluor® 546 dye, MB™ 543 dye contains an additional, negatively charged sulfo group (SO3H) to improve water solubility and minimize self-quenching. Characteristic features of MB™ 543 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™ 543 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.

MB™ 543 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern MB™ 543 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Reactivity
Azide, picolyl azide
Abs/Em Maxima
543/566 nm
Extinction coefficient
105,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 546, CF® 543, TAMRA
Molecular weight
1078.24
Storage conditions
-20C
Shipping conditions
Ambient temperature