MB 488 NHS ester

Catalog#: 1601-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1601-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1601-25
Unit size: 25 mg
$1,395.00
In stock
Catalog#: 1601-100
Unit size: 100 mg
$2,495.00
In stock

MB™ 488 is a very hydrophilic, water-soluble green emitting dye. Structurally related to Alexa Fluor® 488 dye, MB™ 488 dye is a next step in improving water solubility, brightness and minimizing self-quenching. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and very high photostability. MB™ 488 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally MB™ 488 is an excellent dye for flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many other applications.

The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

Reactivity
Primary amine
Abs/Em Maxima
501/524 nm
Extinction coefficient
86,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight
752.69
Storage conditions
-20C
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label