MB™ 488 is a very hydrophilic, water-soluble green emitting dye. Structurally related to Alexa Fluor® 488 dye, MB™ 488 dye contains an additional, negatively charged sulfo group (SO3H) to improve water solubility and minimize self-quenching. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and very high photostability. MB™ 488 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally MB™ 488 is an excellent dye for flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many other applications.
MB 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection (MB™ 488 DBCO) is also available for application where the presence of copper is not acceptable.