IR 750 Maleimide

Catalog#: 1557-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1557-5
Unit size: 5 mg
$295.00
In stock
Catalog#: 1557-25
Unit size: 25 mg
$1,195.00
In stock
Catalog#: 1557-100
Unit size: 100 mg
$2,795.00
In stock

IR 750 Maleimide (IRDye® 750 Maleimide analog) is a water-soluble, bright, near-IR fluorescent dye that is spectrally similar to other 750 dyes used for flow cytometry, microscopy and other applications. IR 750 conjugates ideally suited for the 633 nm laser lines or dye-pumped laser. A significant advantage to using long wavelength dyes such as Cy7® Dye or Alexa Fluor® 750 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. IR 750 conjugates are is pH-insensitive over a wide molar range, helping ensure that you get stable signals in imaging and flow cytometry applications.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

Reactivity
Thiol (Cys)
Abs/Em Maxima
756/776 nm
Extinction coefficient
270,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Cy7, Alexa Fluor® 750, IRDye® 750
Molecular weight
1137.36
Storage conditions
-20C
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

IRDye® is a trademark of LI-COR Biotechnology.