IR 750 Alkyne

Catalog#: 1560-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1560-5
Unit size: 5 mg
$295.00
In stock
Catalog#: 1560-25
Unit size: 25 mg
$1,195.00
In stock
Catalog#: 1560-100
Unit size: 100 mg
$2,795.00
In stock

IR 750 Alkyne (IRDye® 750 Alkyne analog) is a water-soluble, bright, near-IR fluorescent probe that is pH-insensitive from pH 4 to pH 10. Its excitation band is ideally suited for the 633 nm laser lines or dye-pumped laser. IR 750 dye is spectrally similar to other 750 dyes used for flow cytometry, microscopy and other applications. A significant advantage to using long wavelength dyes such as Cy7® Dye or Alexa Fluor® 750 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

The near-IR fluorescent IR 750 Alkyne can be reacted with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole bond. Click chemistry-based detection of azide-tagged biomolecules in complex biological samples can be done with unprecedented sensitivity due to extremely low background.

A probe for copper-less detection azide-tagged biomolecules, IR 750 DBCO, is also available for application where the presence of copper is not acceptable.

Reactivity
Azide, picolyl azide
Abs/Em Maxima
756/776 nm
Extinction coefficient
270,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Cy7, Alexa Fluor® 750, IRDye® 750
Molecular weight
1039.27
Storage conditions
-20C
Shipping conditions
Ambient temperature

IRDye® is a trademark of LI-COR Biotechnology.