IR 650 Picolyl Azide

Catalog#: 1504-1
Unit size: 1 mg
$139.00
In stock
Catalog#: 1504-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1504-25
Unit size: 25 mg
$1,295.00
In stock
Catalog#: 1504-100
Unit size: 100 mg
$2,895.00
In stock

IR 650 Picolyl Azide (IRDye® 650 Azide analog) is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The rate of CuAAC reaction depends on concentrations of all reagents, including copper, thus the raise of the effective copper concentration at the reaction site dramatically increase the rate of CuAAC reaction without the need to increase concentration of azide reagent and copper.

In addition, the use of IR 650 Picolyl Azide instead of conventional IR 650 Azide allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.

In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.

Reactivity
Alkyne, cyclooctyne
Abs/Em Maxima
651/668 nm
Extinction coefficient
230,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Cy5, Alexa Fluor® 647, DyLight® 649,
Molecular weight
1137.32
Storage conditions
-20C
Shipping conditions
Ambient temperature

IRDye® is a trademark of LI-COR Biotechnology.