IR 650 Azide

Catalog#: 1503-1
Unit size: 1 mg
$95.00
In stock
Catalog#: 1503-5
Unit size: 5 mg
$295.00
In stock
Catalog#: 1503-25
Unit size: 25 mg
$895.00
In stock
Catalog#: 1503-100
Unit size: 100 mg
$2,795.00
In stock

IR 650 Azide (IRDye® 650 Azide analog) is a water-soluble, bright, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as IR 650, Cy5® Dye or Alexa Fluor® 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

IR 650 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

For the detection of low abundance targets or where significant increase in signal intensity is desired please consider using next generation azides probes containing an internal copper-chelating motif.

Reactivity
Alkyne, cyclooctyne
Abs/Em Maxima
651/668 nm
Extinction coefficient
230,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Cy5, Alexa Fluor® 647, DyLight® 649,
Molecular weight
1017.21
Storage conditions
-20C
Shipping conditions
Ambient temperature

IRDye® is a trademark of LI-COR Biotechnology.