AMCA (7-amino-4-methyl-3-coumarinacetic acid) is a blue fluorescent dye that is a non-sulfonated version of the Alexa Fluor® 350 dye. It is often used as a contrasting probe for double - and triple - labeling in immunofluorescence microscopy, arrays, and in situ hybridization. The desirable properties of AMCA dyes include a relatively large Stoke’s shift and resistance to photobleaching.
Fmoc Lys(AMCA) OH is a building block for in-sequence peptide labeling by AMCA dye. It allows incorporation of fluorescent tag into a peptide chain using standard Fmoc peptide solid phase synthesis. This reagent is supplied as Boc protected derivative to ensure compatibility with Fmoc solid phase peptide synthesis. Spacers of different lengths are available upon request.