Cy5 DBCO is a water-soluble, bright, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AFDye 647 over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.
Cy5 DBCO is a bright, far-red-fluorescent, pH insensitive azide-reactive probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. Cy5 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Cy5 DBCO is an ideal alternative to copper requiring fluorescent alkynes.