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Cy5 Alkyne

Unit Size
Price (USD)
Cat.#: 1326-1
Unit size: 1 mg
in stock
Cat.#: 1326-5
Unit size: 5 mg
in stock
Cat.#: 1326-25
Unit size: 25 mg
in stock
Cat.#: 1326-100
Unit size: 100 mg
in stock

Cy5 Alkyne (also sold under the Sulfo-Cyanine5 Alkyne name) is a water-soluble, bright, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AF 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

Cy5 Alkyne is a bright, far-red-fluorescent, pH insensitive alkyne-activated probe routinely used for imaging of azide-containing biomolecules. Cy5 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection (Cy5 DBCO) is also available for application where the presence of copper is not acceptable.

Azide, picolyl azide
Abs/Em Maxima
648/671 nm
Extinction coefficient
250,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 647, DyLight® 649,
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature