Cy5.5 NHS ester

Catalog#

Unit Size

Price (USD)

Availability

Qty

Cat.#: 1341-1

Unit size: 1 mg

$129.00

in stock

Cat.#: 1341-5

Unit size: 5 mg

$345.00

in stock

Cat.#: 1341-25

Unit size: 25 mg

$945.00

in stock

Cat.#: 1341-100

Unit size: 100 mg

$2,420.00

in stock

Cy5.5 NHS Ester (also sold under sulfo-Cyanine5.5 NHS Ester name) is a bright and photostable near-IR dye that is spectrally similar to Alexa Fluor® 680, DyLight® 680, and IRDye® 680 dye. Cy5.5 NHS Ester is completely water-soluble and often a reagent of choice for labeling antibodies, peptides, and proteins that are sensitive toward organic co-solvents. Cy5.5 conjugates are pH insensitive from pH 4 to pH 10 and produce minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.

The NHS ester (succinimidyl ester) of Cy5.5 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Specifications
Structure
Abs/Em Spectra
Protein Labeling Calculator
Documents

Reactivity

Primary amine

Abs/Em Maxima

675/694 nm

Extinction coefficient

190,000 cm-1M-1

Solubility

Water, DMSO, DMF

Spectrally similar dyes

Alexa Fluor® 680, DyLight® 680, IRDye® 680

Molecular weight

1000.09

Storage conditions

-20C

Shipping conditions

Ambient temperature

Protein name

Protein molecular weight (Da)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) *
* Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)