Cy5.5 NHS ester

Catalog#: 1341-1
Unit size: 1 mg
$110.00
In stock
Catalog#: 1341-5
Unit size: 5 mg
$295.00
In stock
Catalog#: 1341-25
Unit size: 25 mg
$690.00
In stock
Catalog#: 1341-100
Unit size: 100 mg
$1,850.00
In stock

Cy5.5 NHS Ester (also sold under sulfo-Cyanine5.5 NHS Ester name) is a bright and photostable near-IR dye that is spectrally similar to Alexa Fluor® 680, DyLight® 680, and IRDye® 680 dye. Cy5.5 NHS Ester is completely water-soluble and often a reagent of choice for labeling antibodies, peptides, and proteins that are sensitive toward organic co-solvents. Cy5.5 conjugates are pH insensitive from pH 4 to pH 10 and produce minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.

The NHS ester (succinimidyl ester) of Cy5.5 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Reactivity
Primary amine
Abs/Em Maxima
675/694 nm
Extinction coefficient
190,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 680, DyLight® 680, IRDye® 680
Molecular weight
1014.12
Storage conditions
-20C
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Cy®Dye is a trademark of GE Healthcare.