Cy5.5 Azide

Catalog#: 1344-1
Unit size: 1 mg
$110.00
In stock
Catalog#: 1344-5
Unit size: 5 mg
$295.00
In stock
Catalog#: 1344-25
Unit size: 25 mg
$690.00
In stock
Catalog#: 1344-100
Unit size: 100 mg
$1,850.00
In stock

Cy5.5 Azide (also sold under sulfo-Cyanine5.5 Azide name) is a bright and photostable near-IR dye that is spectrally similar to Alexa Fluor® 680, DyLight 680™, and IRDye® 680 dye. Cy5.5 Azide is water-soluble, hydrophilic dye often a reagent of choice for assay where minimal non-specific binding is required. Cy5.5 Azide is pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.

Cy5.5 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

For the detection of low abundance targets or where significant increase in signal intensity is desired please consider using next generation azide probes containing an internal copper-chelating motif.

Reactivity
Alkyne, cyclooctyne
Abs/Em Maxima
675/694 nm
Extinction coefficient
190,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 680, DyLight® 680, IRDye® 680
Molecular weight
999.15
Storage conditions
-20C
Shipping conditions
Ambient temperature

Cy®Dye is a trademark of GE Healthcare.