Cy3 Azide

Catalog#: 1304-1
Unit size: 1 mg
$89.00
In stock
Catalog#: 1304-5
Unit size: 5 mg
$249.00
In stock
Catalog#: 1304-25
Unit size: 25 mg
$645.00
In stock
Catalog#: 1304-100
Unit size: 100 mg
$1,795.00
In stock

Cy3 Azide (also sold under Sulfo-Cyanine3 Azide) is a bright, water-soluble, and pH insensitive from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.

Cy3 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

For the detection of low abundance targets or where significant increase in signal intensity is desired please consider using next generation azides probes containing an internal copper-chelating motif.

Reactivity
Alkyne, cyclooctyne
Abs/Em Maxima
555/572 nm
Extinction coefficient
150,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Cy3, DyLight® 555, Alexa Fluor® 555
Molecular weight
774.86
Storage conditions
-20C
Shipping conditions
Ambient temperature

Cy®Dye is a trademark of GE Healthcare.