Carboxyrhodamine 110 (also known as Rhodamine Green™ Carboxylic Acid) is the nonsulfonated analog of the Alexa Fluor® 488 dye. The amine-reactive Carboxyrhodamine 110 LC can be used to create bright and photostable green-fluorescent bioconjugates with excitation/emission maxima ~502/527 nm. For many applications, the dye is preferred over carboxyfluorescein or FITC because of its exceptional photostability and fluorescence insensitivity to pH (4-9).
This reagent is supplied as Boc protected derivative to ensure compatibility with Fmoc solid phase peptide synthesis. The hydrophilic spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group imparts water solubility of the labeled peptide resulting in less aggregation when stored in aqueous solution. Spacers of different lengths are available upon request.