Carboxyrhodamine 110 (also known as Rhodamine Green™ Carboxylic Acid) is the nonsulfonated analog of the Alexa Fluor® 488 dye. The amine-reactive Carboxyrhodamine 110 LC can be used to create bright and photostable green-fluorescent bioconjugates with excitation/emission maxima ~502/527 nm. For many applications, the dye is preferred over carboxyfluorescein or FITC because of its exceptional photostability and fluorescence insensitivity to pH (4-9).
This reagent is supplied as Boc protected derivative to ensure compatibility with Fmoc solid phase peptide synthesis. Medium length, hydrophobic spacer arm (total length is 22.4 angstroms) provides an adequate separation between the dye and peptide. Spacers of different lengths are available upon request.