5(6)-Carboxyrhodamine 110 Azide

Catalog#: 1204-1
Unit size: 1 mg
$79.00
In stock
Catalog#: 1204-5
Unit size: 5 mg
$179.00
In stock
Catalog#: 1204-25
Unit size: 25 mg
$529.00
In stock
Catalog#: 1204-100
Unit size: 100 mg
$1,295.00
In stock

5(6)-Carboxyrhodamine 110 Azide5(6)-Carboxyrhodamine 110 Azide (also known as Rhodamine Green) is the nonsulfonated analog of the Alexa Fluor® 488 dye with excitation/emission maxima ~502/527 nm. Carboxyrhodamine 110 is bright and exceedingly photostable and pH-insensitive from pH 4 to pH 10 green-fluorescent probe. Unlike Alexa Fluor® 488, 5(6)-Carboxyrhodamine 110 is stable at highly alkaline conditions. This probe can be used with the 488 nm line of argon-ion laser and standard FITS filter set.

5(6)-Carboxyrhodamine 110 Azide is green-fluorescent probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

For the detection of low abundance targets or where significant increase in signal intensity is desired please consider using next generation azides probes containing an internal copper-chelating motif.

Reactivity
Alkynes, cyclooctynes
Abs/Em Maxima
502/527 nm
Extinction coefficient
76,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight
575.59
Storage conditions
-20C
Shipping conditions
Ambient temperature