Biotin-NHS Ester is an amine-reactive building block used for modification of amine-containing molecule in organic media. NHS-activated biotin is water-insoluble reagent, thus requires an organic solvent such as DMSO or DMF to be dissolved. Short spacer arm adds minimal mass to modified molecules.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.