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AMCA LC Acid

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1680-100
Unit size: 100 mg
$95.00
in stock
Cat.#: 1680-500
Unit size: 500 mg
$295.00
in stock
Cat.#: 1680-1G
Unit size: 1000 mg
$520.00
in stock
Cat.#: 1680-5G
Unit size: 5 g
$1,910.00
in stock

AMCA (7-amino-4-methyl-3-coumarinacetic acid) is a blue fluorescent dye that is a non - sulfonated version of the Alexa Fluor® 350 dye. It is often used as a contrasting probe for double -  and triple - labeling in immunofluorescence microscopy, arrays, and in situ hybridization. The desirable properties of AMCA dyes include a relatively large Stoke’s shift and resistance to photobleaching.

This reagent is supplied as Boc protected derivative to ensure compatibility with Fmoc solid phase peptide synthesis. Medium length, hydrophobic spacer arm (total length is 22.4 angstroms) provides an adequate separation between the dye and peptide. Spacers of different lengths are available upon request.

Reactivity
Primary amines (needs activation)
Abs/Em Maxima
345/450 nm
Extinction coefficient
19,000 cm-1M-1
Solubility
DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 350, AMCA, DyLight® 350
Molecular weight
446.50
Storage conditions
-20C
Shipping conditions
Ambient temperature