AMCA Acid

Catalog#: 1235-25
Unit size: 25 mg
$95.00
In stock
Catalog#: 1235-100
Unit size: 100 mg
$195.00
In stock
Catalog#: 1235-1000
Unit size: 1000 mg
$595.00
In stock

AMCA Acid (7-amino-4-methylcoumarin-3-acetic acid) is one of the most popular blue fluorescent tagging molecules. Often used as contrasting probes for double- and triple-labeling in immunofluorescence microscopy, arrays and in situ hybridization. The desirable properties of AMCA dyes include a relatively large Stoke’s shift and resistance to photobleaching.

AMCA Acid is a reagent of choice for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, for example HATU. AMCA Acid is also often used for control experiments, and for calibration.

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Reactivity
Primary amines (needs activation)
Abs/Em Maxima
345/450 nm
Extinction coefficient
19,000 cm-1M-1
Solubility
DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 350, AMCA, DyLight® 350
Molecular weight
223.22
Storage conditions
-20C
Shipping conditions
Ambient temperature