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Catalog#: 1235-25
Unit size: 25 mg
In stock
Catalog#: 1235-100
Unit size: 100 mg
In stock
Catalog#: 1235-1000
Unit size: 1000 mg
In stock

AMCA Acid (7-amino-4-methylcoumarin-3-acetic acid) is one of the most popular blue fluorescent tagging molecules. Often used as contrasting probes for double- and triple-labeling in immunofluorescence microscopy, arrays and in situ hybridization. The desirable properties of AMCA dyes include a relatively large Stoke’s shift and resistance to photobleaching.

AMCA Acid is a reagent of choice for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, for example HATU. AMCA Acid is also often used for control experiments, and for calibration.

Click the link to see our other classic fluorescent dyes.

Primary amines (needs activation)
Abs/Em Maxima
345/450 nm
Extinction coefficient
19,000 cm-1M-1
Spectrally similar dyes
Alexa Fluor® 350, AMCA, DyLight® 350
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature