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AFDye 647 NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1121-1
Unit size: 1 mg
$129.00
in stock
Cat.#: 1121-5
Unit size: 5 mg
$395.00
in stock
Cat.#: 1121-25
Unit size: 25 mg
$1,295.00
in stock
Cat.#: 1121-100
Unit size: 100 mg
$2,495.00
in stock

AFDye™ 647 (Alexa Fluor ® 647 analog) is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AFDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with AFDye™647 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. AFDye™ 647 conjugates are often a probe of choice for detection of low abundance targets.

AFDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Reactivity
Primary amine
Abs/Em Maxima
649/671 nm
Extinction coefficient
270,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Cy5, DyLight® 649,
Molecular weight
970.10 (protonated), 1273.68 (triethylammonium salt)
Storage conditions
-20C
Shipping conditions
Ambient temperature

spectrum

Enter only one — either volume or amount of protein to be labeled
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