AFDye 647 Maleimide

Catalog#: 1122-1
Unit size: 1 mg
In stock
Catalog#: 1122-5
Unit size: 5 mg
In stock
Catalog#: 1122-25
Unit size: 25 mg
In stock
Catalog#: 1122-100
Unit size: 100 mg
In stock

Alexa Fluor 647 Maleimide (Alexa Fluor ® 647 Maleimide analog) is a water-soluble, bright, far-red-fluorescent dye and is spectrally similar to other 650 dyes that are used for flow cytometry, microscopy and other applications. AFDye™ 647 conjugates ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AFDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

AFDye™ 647 dye structurally is very similar and spectrally is identical to Invitrogen’s Alexa Fluor® 647 C2 Maleimide

Thiol (Cys)
Abs/Em Maxima
649/671 nm
Extinction coefficient
270,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Cy5, DyLight® 649,
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature

Available upon request

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.