AFDye™ 594 NHS Ester (Alexa Fluor 594 NHS Ester) is the most popular tool for modifying proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules with AFDye™ 594 label. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond. AFDye™ 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
AFDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AFDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
AFDye™ 594 NHS Ester structurally is identical to Invitrogen’s Alexa Fluor® 594 NHS Ester