AFDye 594 NHS ester

Catalog#: 1101-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1101-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1101-25
Unit size: 25 mg
$1,295.00
In stock
Catalog#: 1101-100
Unit size: 100 mg
$2,495.00
In stock

AFDye™ 594 NHS Ester (Alexa Fluor 594 NHS Ester) is the most popular tool for modifying proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules with AFDye™ 594 label. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond. AFDye™ 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

AFDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AFDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 594 NHS Ester structurally is identical to Invitrogen’s Alexa Fluor® 594 NHS Ester

Reactivity
Primary amine
Abs/Em Maxima
590/617 nm
Extinction coefficient
92,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 594, CF™ 594, DyLight® 594
Molecular weight
819.85
Storage conditions
-20C
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.