AFDye 555 NHS ester

Catalog#: 1166-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1166-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1166-25
Unit size: 25 mg
$1,295.00
In stock
Catalog#: 1166-100
Unit size: 100 mg
$2,495.00
In stock

AFDye™ 555 NHS Ester is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AFDye™ 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. Proteins can be labeled with AFDye 555 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. AFDye™ 555 conjugates ideally suited for detection of low abundance targets.

The NHS Ester reacts specifically and efficiently with a primary amine at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

AFDye™ 555 is spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.

Reactivity
Primary amine
Abs/Em Maxima
555/572 nm
Extinction coefficient
155,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Cy5, DyLight® 649,
Molecular weight
1247.64
Storage conditions
-20C
Shipping conditions
Ambient temperature

Available upon request

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.