AZDye 555 NHS ester

Catalog#

Unit Size

Price (USD)

Availability

Qty

Cat.#: 1166-1

Unit size: 1 mg

$149.00

in stock

Cat.#: 1166-5

Unit size: 5 mg

$465.00

in stock

Cat.#: 1166-25

Unit size: 25 mg

$1,495.00

in stock

Cat.#: 1166-100

Unit size: 100 mg

$2,790.00

in stock

AZDye™ 555 NHS Ester is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AZDye™ 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. Proteins can be labeled with AZDye 555 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. AZDye™ 555 conjugates ideally suited for detection of low abundance targets.

The NHS Ester reacts specifically and efficiently with a primary amine at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

Specifications
Structure
Abs/Em Spectra
Protein Labeling Calculator
Documents

Reactivity

Primary amine

Abs/Em Maxima

555/572 nm

Extinction coefficient

155,000 cm-1M-1

Solubility

Water, DMSO, DMF

Spectrally similar dyes

Cy3, DyLight® 550, Alexa Fluor® 555

Molecular weight

930.04 (protonated)

Storage conditions

-20C

Shipping conditions

Ambient temperature

Protein name

Protein molecular weight (Da)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) *
* Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)