AFDye 555 Maleimide

Catalog#: 1168-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1168-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1168-25
Unit size: 25 mg
$1,295.00
In stock
Catalog#: 1168-100
Unit size: 100 mg
$2,495.00
In stock

AFDye™ 555 Maleimide is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AFDye™ 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. Proteins can be labeled with AFDye™ 555 dye at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. AF 555 conjugates ideally suited for detection of low abundance targets.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

AFDye™ 555 is spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.

Reactivity
Thiol (Cys)
Abs/Em Maxima
556/572 nm
Extinction coefficient
158,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Cy5, DyLight® 649,
Molecular weight
1272.70
Storage conditions
-20C
Shipping conditions
Ambient temperature

Available upon request

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Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.