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AFDye 680 NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1721-1
Unit size: 1 mg
$139.00
in stock
Cat.#: 1721-5
Unit size: 5 mg
$435.00
in stock
Cat.#: 1721-25
Unit size: 25 mg
$1,350.00
in stock
Cat.#: 1721-100
Unit size: 100 mg
$2,750.00
in stock

AFDye 680 (Alexa Fluor ® 680 equivalent) is a bright and photostable near-IR dye that is spectrally identical to Alexa Fluor® 680 and IRDye® 680RD. AFDye 680 is often used for small animal imaging applications in the 700 nm wavelength and provides excellent clearance profiles in animals. Flow cytometry is another common application for this dye. AFDye 680 ideally suited for the 633 nm laser line. This near-IR fluorescent dye is water soluble and pH-insensitive from pH 4 to pH 10. AFDye 680 molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The long wavelength emission allows for detection in complex samples with auto-fluorescent background signals.

The NHS ester (succinimidyl ester) of AFDye 680 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Reactivity
Primary amine
Abs/Em Maxima
678/701 nm
Extinction coefficient
185,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 680, DyLight® 680, IRDye® 680
Molecular weight
924.21
Storage conditions
-20C
Shipping conditions
Ambient temperature

spectrum

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Thermo Fisher Scientific.