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AFDye 647 TPF Ester

Catalog#: 1129-01
Unit size: 3 x 100 μg
$149.00
In stock
Catalog#: 1129-1
Unit size: 1 mg
$149.00
In stock
Catalog#: 1129-5
Unit size: 5 mg
$445.00
In stock
Catalog#: 1129-25
Unit size: 25 mg
$1,495.00
In stock

AFDye™ 647 (Alexa Fluor ® 647 analog) is a water-soluble, bright, far-red fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AFDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

TFP (tetrafluorophenyl) ester is an amine-reactive activated ester that reacts with primary amines of biomolecules in the same way as the succinimidyl ester (SE or NHS-ester) to form a stable amide bond. The major advantage of TFP esters over the succinimidyl ester is much improved resistance to spontaneous hydrolysis during conjugation reactions resulting in more efficiency and better reproducible labeling of biopolymers. TFP esters are stable for several hours at the basic pH typically used for reactions–far outlasting succinimidyl esters.

AFDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Reactivity
Primary amine
Abs/Em Maxima
649/671 nm
Extinction coefficient
270,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Cy5, DyLight® 649,
Molecular weight
1021.09 (protonated)
Storage conditions
-20C
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.