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AFDye 594 TFP Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1111-1
Unit size: 1 mg
$129.00
in stock
Cat.#: 1111-5
Unit size: 5 mg
$395.00
in stock
Cat.#: 1111-25
Unit size: 25 mg
$1,295.00
in stock
Cat.#: 1111-100
Unit size: 100 mg
$2,795.00
in stock

AFDye™ 594 TFP Ester (Alexa Fluor 594 TFP Ester) is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye often used for modifying proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules with AFDye™ 594 label. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond. AFDye™ 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

TFP (tetrafluorophenyl) ester is an amine-reactive activated ester that reacts with primary amines of biomolecules in the same way as the succinimidyl ester (SE or NHS-ester) to form a stable amide bond. The major advantage of TFP esters over the succinimidyl ester is much improved resistance to spontaneous hydrolysis during conjugation reactions resulting in more efficiency and better reproducible labeling of biopolymers. TFP esters are stable for several hours at the basic pH typically used for reactions–far outlasting succinimidyl esters.

Reactivity
Primary amine
Abs/Em Maxima
590/617 nm
Extinction coefficient
92,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 594, CF™ 594, DyLight® 594
Molecular weight
870.84
Storage conditions
-20C
Shipping conditions
Ambient temperature

spectrum

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Thermo Fisher Scientific.