AFDye 488 Hydroxylamine (aminooxy) reacts with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. AFDye 568 Hydroxlamine often used as membrane-impermeant, aldehyde-fixable cell tracers as well as reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins.
AFDye™ 568 is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.
AFDye™ 568 structurally is identical to Alexa Fluor® 568 dye sold by ThermoFisher Scientific.