Alexa Fluor 555 DBCO (Alexa Fluor® 555 sDIBO replacement) reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AF™ 555 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AFDye™ 555 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 555 and 572 nm, respectively. It can be used with the 532 nm and 555 nm laser lines. The brightness and photostability of this dye are best suited for direct imaging of low-abundance targets.
AFDye™ 555 is spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.