AFDye 555 Azide

Catalog#: 1170-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1170-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1170-25
Unit size: 25 mg
$1,495.00
In stock
Catalog#: 1170-100
Unit size: 100 mg
$2,895.00
In stock

AFDye™ 555 Azide (Alexa Fluor® 555 Azide replacement) is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AFDye™ 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. AF 555 conjugates ideally suited for detection of low abundance targets.

AFDye™ 555 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

AFDye™ 555 is spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.

Reactivity
Alkyne, cyclooctyne
Abs/Em Maxima
555/572 nm
Extinction coefficient
155,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Cy3, DyLight® 555, Alexa Fluor® 555
Molecular weight
1232.68
Storage conditions
-20C
Shipping conditions
Ambient temperature

Available upon request

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Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AF Dyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.