AFDye 555 Alkyne

Catalog#: 1172-1
Unit size: 1 mg
$139.00
In stock
Catalog#: 1172-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1172-25
Unit size: 25 mg
$1,295.00
In stock
Catalog#: 1172-100
Unit size: 100 mg
$2,695.00
In stock

AFDye™ 555 Alkyne (Alexa Fluor 555 Alkyne replacement) is a water-soluble, bright, orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets.

AFDye™ 555 Alkyne is a very bright, pH insensitive alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AFDye™ 555 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection (AFDye™ 555 DBCO) is also available for application where the presence of copper is not acceptable.

AFDye™ 555 is spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.

Reactivity
Azide, picolyl azide
Abs/Em Maxima
555/572 nm
Extinction coefficient
155,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Cy3, DyLight® 555, Alexa Fluor® 555
Molecular weight
884.06
Storage conditions
-20C
Shipping conditions
Ambient temperature

Available upon request

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AF Dyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.