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AFDye 488 TFP ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1026-100μg
Unit size: 3 x 100 μg
$129.00
in stock
Cat.#: 1026-1
Unit size: 1 mg
$139.00
in stock
Cat.#: 1026-5
Unit size: 5 mg
$395.00
in stock
Cat.#: 1026-25
Unit size: 25 mg
$1,295.00
in stock

AFDye™ 488 TFP Ester (Alexa Fluor® 488 TFP Ester equivalent) is an amine reactive, green-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond. AFDye™ 488 conjugates are highly photostable dyes with very high fluorescence quantum yields.

TFP (tetrafluorophenyl) ester is an amine-reactive activated ester that reacts with primary amines of biomolecules in the same way as the succinimidyl ester (SE or NHS-ester) to form a stable amide bond. The major advantage of TFP esters over the succinimidyl ester is better resistance to spontaneous hydrolysis during conjugation reactions, which results in more efficient and reproducible labeling of biopolymers. TFP esters are stable for several hours at the basic pH typically used for reactions–far outlasting succinimidyl esters.

AFDye™ 488 dye structurally is identical to Invitrogen’s Alexa Fluor® 488 dye.

Reactivity
Primary amines
Abs/Em Maxima
494/517 nm
Extinction coefficient
73,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight
682.53 (protonated)
Storage conditions
-20C
Shipping conditions
Ambient temperature

spectrum

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label