AFDye 488 Hydroxylamine

Unit Size
Price (USD)
Cat.#: 1016-1
Unit size: 1 mg
in stock
Cat.#: 1016-5
Unit size: 5 mg
in stock
Cat.#: 1016-25
Unit size: 25 mg
in stock

AFDye 488 Hydroxylamine (aminooxy) reacts with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. AFDye 488 Hydroxlamine often used as membrane-impermeant, aldehyde-fixable cell tracers as well as reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins.

AFDye™ 488 (Alexa Fluor® 488 equivalent) is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AFDye™ 488 dyes are best suited to direct imaging of low-abundance targets.

Aldehyde, ketone
Abs/Em Maxima
594/517 nm
Extinction coefficient
73,000 cm-1M-1
Water, DMSO, DMF, MeOH
Spectrally similar dyes
Alexa Fluor® 488, CF™ 488
Molecular weight
606.58 (protonated)
Storage conditions
Shipping conditions
Ambient temperature

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific.