AFDye 488 Hydroxylamine (aminooxy) reacts with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. AFDye 488 Hydroxlamine often used as membrane-impermeant, aldehyde-fixable cell tracers as well as reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins.
AFDye™ 488 (Alexa Fluor® 488 equivalent) is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AFDye™ 488 dyes are best suited to direct imaging of low-abundance targets.