AFDye™ 488 Hydrazide (Alexa Fluor® 488 Hydrazide equivalent) is a bright, green-fluorescent dye that often used for labeling aldehydes or ketones in polysaccharides or glycoproteins. AFDye™ 488 Hydrazide is useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores.
AFDye™ 488 (Alexa Fluor® 488 equivalent) is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AFDye™ 488 dyes are best suited to direct imaging of low-abundance targets.