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AFDye 488 Hydrazide

Unit Size
Price (USD)
Cat.#: 1017-1
Unit size: 1 mg
in stock
Cat.#: 1017-5
Unit size: 5 mg
in stock
Cat.#: 1017-25
Unit size: 25 mg
in stock

AFDye™ 488 Hydrazide (Alexa Fluor® 488 Hydrazide equivalent) is a bright, green-fluorescent dye that often used for labeling aldehydes or ketones in polysaccharides or glycoproteins. AFDye™ 488 Hydrazide is useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores.

AFDye™ 488 (Alexa Fluor® 488 equivalent) is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AFDye™ 488 dyes are best suited to direct imaging of low-abundance targets.

Aldehyde, ketone,
Abs/Em Maxima
494/517 nm
Extinction coefficient
73,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight
548.50 (protonated)
Storage conditions
Shipping conditions
Ambient temperature

Alexa Fluor® is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes™ are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.