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AFDye 488 Acid

Unit Size
Price (USD)
Cat.#: 1012-5
Unit size: 5 mg
in stock
Cat.#: 1012-25
Unit size: 25 mg
in stock
Cat.#: 1012-100
Unit size: 100 mg
in stock

AFDye™ 488 is a water-soluble, highly photostable dye with a very high fluorescence quantum yield. AFDye™ 488 is green-emitting dye with excitation ideally suited for the 488 nm line. Its fluorescence is pH independent from pH 4 to pH 10.

AFDye™ 488 Acid (Alexa Fluor® 488 Acid equivalent)  often used for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), and STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for the non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, e.g. HATU.

AFDye™ 488 dye structurally is identical to Alexa Fluor® 488 dye.

Primary amines
Abs/Em Maxima
494/517 nm
Extinction coefficient
73,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight
534.47 (protonated)
Storage conditions
Shipping conditions
Ambient temperature

Alexa Fluor® is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes™ are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.