AFDye 430 NHS Ester

Catalog#: 1031-5
Unit size: 5 mg
In stock
Catalog#: 1031-25
Unit size: 25 mg
In stock
Catalog#: 1031-100
Unit size: 100 mg
In stock

AFDye™ 430 NHS Ester is a water-soluble, green-fluorescent dye that often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM. It’s excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AF 405 conjugates are pH-insensitive from pH 4 to pH10.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with AFDye™ 430 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.

AFDye™ 430 dye structurally is identical to Invitrogen’s Alexa Fluor® 430

Alkynes, cyclooctynes
Abs/Em Maxima
430/539 nm
Extinction coefficient
15,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 430
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.