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AFDye 430 NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1031-5
Unit size: 5 mg
$179.00
in stock
Cat.#: 1031-25
Unit size: 25 mg
$395.00
in stock
Cat.#: 1031-100
Unit size: 100 mg
$1,295.00
in stock

AFDye™ 430 NHS Ester is a water-soluble, green-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 conjugates are pH-insensitive from pH 4 to pH 10.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with AFDye™ 430 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.

AFDye™ 430 is structurally identical to Invitrogen’s Alexa Fluor® 430 Dye.

Reactivity
Alkynes, cyclooctynes
Abs/Em Maxima
430/539 nm
Extinction coefficient
15,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 430
Molecular weight
585.19
Storage conditions
-20C
Shipping conditions
Ambient temperature

spectrum

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label