AFDye 350 Picolyl Azide

Catalog#: 1011-1
Unit size: 1 mg
$135.00
In stock
Catalog#: 1011-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1011-25
Unit size: 25 mg
$1,295.00
In stock
Catalog#: 1011-100
Unit size: 100 mg
$2,495.00
In stock

AFDye™ 350 Picolyl Azide (Alexa Fluor® 350 Picolyl Azide equivalent) is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The rate of CuAAC reaction depends on concentrations of all reagents, including copper, thus the raise of the effective copper concentration at the reaction site dramatically increase the rate of CuAAC reaction without the need to increase concentration of azide reagent and copper.

In addition, the use of AFDye™ 350 Picolyl Azide instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.

In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.is a blue-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AFDye™ 350 dye structurally is identical to Invitrogen’s Alexa Fluor® 350

Reactivity
Alkynes, cyclooctynes
Abs/Em Maxima
346/445 nm
Extinction coefficient
19,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 350, AMCA, DyLight® 350
Molecular weight
515.50
Storage conditions
-20C
Shipping conditions
Ambient temperature

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AF Dyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.