AZDye 350 Picolyl Azide

Unit Size
Price (USD)
Cat.#: 1011-1
Unit size: 1 mg
in stock
Cat.#: 1011-5
Unit size: 5 mg
in stock
Cat.#: 1011-25
Unit size: 25 mg
in stock

AZDye™ 350 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif in order to raise the effective concentration of Cu(I) at the reaction site. The rate of the CuAAC reaction depends on the concentrations of all reagents, including copper, therefore raising the effective copper concentration at the reaction site dramatically increases the rate of the CuAAC reaction without needing to increase the concentration of azide reagent or copper.

In addition, the use of AZDye™ 350 Picolyl Azide instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.

In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This is of special value for the detection of low abundance targets or where significant increase in signal intensity is desired. AZDye™ 350 Picolyl Azide is a blue-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

Alkynes, cyclooctynes
Abs/Em Maxima
346/445 nm
Extinction coefficient
19,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 350, AMCA, DyLight® 350
Molecular weight
Storage conditions
Shipping conditions
Ambient temperature

Alexa Fluor® is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AZDyes™ are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.