AFDye 350 NHS Ester

Catalog#: 1002-1
Unit size: 1 mg
$95.00
In stock
Catalog#: 1002-5
Unit size: 5 mg
$295.00
In stock
Catalog#: 1002-25
Unit size: 25 mg
$895.00
In stock
Catalog#: 1002-100
Unit size: 100 mg
$1,985.00
In stock

AFDye™ 350 NHS Ester (Alexa Fluor® 350 NHS Ester equivalent) is an amine reactive, water-soluble, blue-emitting dye used to specifically and efficiently modify a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of proteins or antibodies (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

AFDye™ 350 is a moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.

AFDye™ 350 NHS Ester is structurally identical to Invitrogen’s Alexa Fluor® 350 NHS Ester.

Reactivity
Primary amines
Abs/Em Maxima
346/445 nm
Extinction coefficient
19,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 350, AMCA, DyLight® 350
Molecular weight
410.35
Storage conditions
-20C
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes™ are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.