AFDye 647 Maleimide

Catalog#: 1122-1
Unit size: 1 mg
$129.00
In stock
Catalog#: 1122-5
Unit size: 5 mg
$395.00
In stock
Catalog#: 1122-25
Unit size: 25 mg
$1,295.00
In stock
Catalog#: 1122-100
Unit size: 100 mg
$2,795.00
In stock

AFDye™ 647 Maleimide is a water-soluble, far-red-fluorescent dye that is spectrally similar to other 650 dyes that are used for flow cytometry, microscopy and other applications. AFDye™ 647 conjugates ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AFDye™ 647 over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

AFDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Reactivity
Thiol (Cys)
Abs/Em Maxima
649/671 nm
Extinction coefficient
270,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Cy5, DyLight® 649,
Molecular weight
995.16 (protonated), 1298.72 (triethylammonium salt)
Storage conditions
-20C
Shipping conditions
Ambient temperature

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.