AFDye 647 DBCO

Catalog#: 1127-1
Unit size: 1 mg
In stock
Catalog#: 1127-5
Unit size: 5 mg
In stock
Catalog#: 1127-25
Unit size: 25 mg
In stock
Catalog#: 1127-100
Unit size: 100 mg
In stock

AFDye 647 DBCO is a water-soluble, pH-insensitive from pH 4 to pH 10 bright, far-red fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AFDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

AFDye™ 647 DBCO is a bright, far-red fluorescent, probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. AFDye™ 647 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 647 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AFDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Azide, picolyl azide
Abs/Em Maxima
648/671 nm
Extinction coefficient
270,000 cm-1M-1
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 647, DyLight® 649,
Molecular weight
1131.36 (protonated)
Storage conditions
Shipping conditions
Ambient temperature

Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. Fluoroprobes is not affiliated with or endorsed by Thermo Fisher Scientific. AFDyes are GENERIC versions of Alexa Fluor® Dyes and ARE NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.