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AFDye 647 Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1124-1
Unit size: 1 mg
$149.00
in stock
Cat.#: 1124-5
Unit size: 5 mg
$465.00
in stock
Cat.#: 1124-25
Unit size: 25 mg
$1,495.00
in stock

AFDye™ 647 Azide (Alexa Fluor 647 Azide analog) is a water-soluble, pH-insensitive from pH 4 to pH 10 bright, far-red fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AFDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

AFDye™ 647 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

For the detection of low abundance targets or where significant increase in signal intensity is desired please consider using next generation azides probes containing an internal copper-chelating motif.

AFDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Reactivity
Alkyne, cyclooctyne
Abs/Em Maxima
648/671 nm
Extinction coefficient
270,000 cm-1M-1
Solubility
Water, DMSO, DMF
Spectrally similar dyes
Alexa Fluor® 647, DyLight® 649,
Molecular weight
Storage conditions
-20C
Shipping conditions
Ambient temperature

spectrum